Sperm chromatin integrity test

Indeed, Marchetti et al. Zeraati for assistance with statistical analyses and S. Interestingly, data demonstrating that toxicants may affect germ cell DNA in the absence of measurable effects on sperm counts, motility or even testicular histology are accumulating. Sperm DNA integrity assessment: Susceptibility of human sperm to in situ DNA denaturation is strongly correlated with DNA strand breaks identified by single cell electrophoresis. Based on various studies of different cases subjected to ICSI program, abnormal sperm factors lead to low fertilization rate and poor embryo quality 14 —

The prepared sperm are incubated with 15 to 20 chemically treated eggs. These are the sperms with denaturated DNA. On the other hand heterogeneous staining of slide and color fading shortly after staining are drawbacks of this technique. The logical questions that arose were: In Comet assay sperm cells are mixed with melted agarose and then placed on a glass slide.

Tests used include the Halosperm kit based on the Sperm Chromatin Decondensation SCD technique in which approximately sperm are characterized as to whether they have expanded nuclei, with five grades of expansion, or no expansion good sperm ; the Tunel assay; the Comet assay; and the Sperm Chromatin Structure Assay SCSA , developed by Dr. The methods used to assess sperm DNA status do not selectively differentiate between clinically important and clinically insignificant fragmentation. Those couples using egg donors or surrogates may also benefit from screening prior to going thru the procedures because the effort and costs are so great. The extent of staining is related to the degree of protamination of mature spermatozoa [ 73 , 74 ].

Many factors are involved in inducing sperm DNA damage, which may result in the male infertility. The applicability of the flow cytometric sperm chromatin structure assay in epidemiological studies. Soc Reprod Fertil Suppl. Exposure to synthetic pyrethroid insecticides is also associated with increased sperm DNA damage, measured as percent DNA in the comet tail Meeker et al. Ostling O, Johanson KJ. DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome.